HuScan® Human Proteome PhIP-Seq

Entire Human Proteome in a Single Tube Assay

HuScan® PhIP-Seq is composed of an entire normal human proteome as defined by the NCBI RefSeq database.

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HuScan® PhIP-Seq Overview

Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a powerful method of multiplexed analysis that combines DNA high-throughput sequencing with next-generation proteomics

PhIP-Seq allows researchers to determine what antibodies were created by an individual’s unique history of immune exposures. PhIP-Seq is the highest-coverage antigen-specific assay yet developed, able to detect antibodies versus virtually any antigen via overlapping long peptides. HuScan® enables simultaneous epitope-level autoantibody profiling versus an entire NCBI human proteome (48,921 proteins and isoforms) via phage-display and immunoprecipitation sequencing.

HuScan Overview

HuScan Technology Overview

Data and technology validated by scientists

"This (VirScan) assay, which uses phage display immunoprecipitation and sequencing, is a sensitive and focused high-comprehensive approach that enables thorough serological profiling of antiviral antibodies in humans and, consequently, the identification of viral exposure throughout the human virome."

Faculty of MedicineResearch Institution (as mentioned in "Human virome profiling identified CMV as the major viral driver of a high accumulation of senescent CD8+ T cells in patients with advanced NSCLC" - Science Advances - November 2023)

Data and technology validated by scientists

"We elected to use the HuProt Microarray because it is an extensive platform that contains over 21,000 unique, individually purified full-length human proteins and protein isoforms in duplicate, covering more than 81% of the proteome."

OncologistSchool of Medicine Research (as mentioned in "Baseline Serum Autoantibody Signatures Predict Recurrence and Toxicity in Melanoma Patients Receiving Adjuvant Immune Checkpoint Blockade" - AACR Clinical Caner Research - September 2022)

Data and technology validated by scientists

"By using (HuProt) protein arrays, we were able to evaluate a broader range of antigens compared to previous investigations."

Senior ScientistBiotech Research (poster presentation at the San Antonio Breast Cancer Symposium 2023)

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HuScan PhIP-Seq Content

An entire NCBI human proteome is included in HuScan™ autoantibody testing. ZIP file containing .csv...

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HuScan: Epitope-level profiling in children with mitochondrial disease reveals limitations in the...

Immunometabolic studies in mice have suggested the importance of oxidative phosphorylation (OXPHOS) in humoral immunity. However, there are important distinctions between murine and human...

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Characterizing Cancer’s Dark Matter, Short-lived Proteins, and Defective Ribosomal Products, Presented...

Short-lived proteins (SLiPs), defective ribosomal products (DRiPs), and Cancer’s dark matter/non-canonical peptides (NCP) are unstable and rapidly degraded, loaded onto MHC and represent a...

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pdf iconTechnology

Overview of HuScan® Human Proteome Phage Display

This video / animation is a short overview of the CDI Labs Canada HuScan® Human Proteome Phage Display product and...

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Overview of Autoantibody Seromics

This video / animation is a short overview of CDI Labs Autoantibody...

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